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Animal Services
 
 
 
 
 
 
 
 
 
Avian

Avian Sexing

Assay Code: AOT-050

Gender determination is crucial to understand the behaviour, evolution ecology, genetics and breeding of avian species. It is also important to protect and sustain endangered avian species by selective breeding or/and producing a hybrid between common and endangered avian species. Based on morphological appearance it is difficult to determine the sex in most of the bird species especially in chicks. The DNA based avian sexing has an accuracy rate greater than 99.9 %.In birds, females are heterogametic (ZW) whereas males are homogametic (ZZ). Hence, in birds, sex is determined at the molecular level by detecting W chromosome linked markers. Uniquely designed primers are used to amplify these sequences by PCR. This method of sexing has become the method of choice for bird owners worldwide.

MBG accepts a variety of sample types (feathers, egg shells, tissues, blood etc) for this service.


METHOD

PCR and Gel Electrophoresis


SPECIMEN TYPE

Recommended specimen type: EDTA Blood (50-100Ál), Feathers (6-12 with roots), Tissue (1g), Fresh egg shells with membrane.

Note: Fresh medium sized feathers are required. Feathers must be freshly plucked out and should have roots. Loose feathers fallen from the bird will not be accepted as sample.
Samples should be individually placed in appropriate labelled containers.


TRANSPORT CONDITION

Sample should be transported at 4°C; except feathers which can be transported at room temperature.


TURN AROUND TIME

Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples: will be reported within half of the minimum test period & will be charged double.


TEST CHARGES



REQUISITION FORM

Avian Species Differentiation

Assay Code: AOT-051

Through Suppression Subtraction hybridization technique, MBG Lab has developed several species specific DNA probes for avian species, namely:

  • Gallus gallus (Chicken)
  • Chlamydotis undulata (Houbara bustard)

Houbara bustard is a wild avian species that is under the threat of extinction. The Houbara populations have declined in recent decades as the result of excessive hunting and illegal trapping. In an effort towards the conservation of the Houbara species, and also due to the scarcity of species specific marker, MBG Lab has designed a few probes that would help us identify Houbara-DNA.


METHOD

Real Time PCR


SPECIMEN TYPE

Recommended specimen type: Tissue (1g), semen (50ul), EDTA blood (50-100Ál).

Note: Fresh medium sized feathers are required. Feathers must be freshly plucked out and should have roots. Loose feathers fallen from the bird will not be accepted as sample.
Samples should be individually placed in appropriate labelled containers.


TRANSPORT CONDITION

Sample should be transported at 4°C.


TURN AROUND TIME

Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples: will be reported within half of the minimum test period & will be charged double.


TEST CHARGES



REQUISITION FORM